Aqueous extract ofLinn. roots potentially attenuates arsenic induced biochemical and genotoxic effects in Wistar rats.
J Tradit Complement Med. 2018 Apr ;8(2):324-334. Epub 2017 Sep 9. PMID: 29736388
Oluwafemi Adeleke Ojo
In Africa, the fruit, leaf, seed and roots ofLinn. are generally used to treat a variety of diseases such as malaria, cancer, and cardiovascular diseases. In this study, we evaluated the protective potentials of aqueous extract ofroots on arsenic-induced biochemical and genotoxic effects in Wistar rats. Rats were induced intraperitoneal with sodium arsenate (dissolved in distilled water at 3 mg/kg body weight) for 21 days and the animals were administered simultaneously with 200 mg/kg body weight vitamin C, 100 and 150 mg/kg body weight of theLinn. root aqueous extract once daily for three weeks. Results obtained reveals that activities of plasma 8-OHdG, serum lipids concentration, atherogenic index (AI), coronary artery index (CRI), aspartate transaminase, alanine transaminase, alkaline phosphatase, total bilirubin levels were elevated significantly ( < 0.05) and catalase, glutathione peroxidase, superoxide dismutase, plasma hematological profile were progressively reduced ( < 0.05) in arsenic-alone exposed rats. Significant increase in the quantity of chromosomal aberrations (CA), micronuclei (MN) frequency, oxidative damages in the bone marrow cells from arsenic alone rats was observed. Though, mitotic index scores in these cells were progressively reduced (p < 0.05). In animals administered with aqueous extract ofroots and vitamin C, the altered parameters were significantly recovered towards the levels observed in normal control rats. These results suggest that aqueousroots preparations might have therapeutic potential as a supplement that can be applied in arsenic poisoning.