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Abstract Title:

Artesunate enhancesγδ T-cell-mediated antitumor activity through augmenting γδ T-cell function and reversing immune escape of HepG2 cells.

Abstract Source:

Immunopharmacol Immunotoxicol. 2018 Apr ;40(2):107-116. Epub 2018 Feb 6. PMID: 29405080

Abstract Author(s):

Peng Qian, Yong-Wen Zhang, Zhong-Hai Zhou, Jun-Quan Liu, Su-Yang Yue, Xiang-Li Guo, Lei-Qing Sun, Xiao-Ting Lv, Jian-Qun Chen

Article Affiliation:

Peng Qian

Abstract:

OBJECTIVE: To explore the effect and mechanism of artesunate onγδ T cell-mediated antitumor immune responses against hepatoma carcinoma cells (HepG2) in vitro.

METHODS: Humanγδ T cells or HepG2 were respectively treated with artesunate, subjected to co-culture as appropriate, and the following assays were subsequently conducted: CCK8 to examine cell viability; LDH release assay to detect the killing effect of γδ T cells on HepG2 cells; flow cytometry to examine theexpression of perforin (PFP) and granzyme B (GraB) of γδ T cells; ELISA to evaluate the levels of TGF-β1 and IL-10 in the collected supernatant of HepG2 cells pretreated with artesunate; and Western blot analysis to examine Fas, FasL, STAT3, p-STAT3 expression of HepG2 cells induced by artesunate.  Results: The results showed that the cytotoxicity effect of γδ T cells pretreated with artesunate on HepG2 cells was augmented via elevating the expression of GraB in γδ T cells. Furthermore, treatment with artesunate reversed the inhibition of HepG2 cells on γδ T cells by reducing the secretion of TGF-β1 in HepG2 cells supernatant and enhanced the antitumor effect of γδ T cells against HepG2 cells through increasing the expression of Fas on HepG2 cells, which may be attributed to the inhibition of STAT3 signaling protein.

CONCLUSION: Artesunate has several mechanisms for augmenting the antitumor immune responses mediated byγδ T cells. These results suggested artesunate may be an efficacious agent in the treatment of hepatocellular carcinoma.

Study Type : In Vitro Study

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