Identification of-dependent biofilm production byclinical isolates and antibiofilm effects of ascorbic acid against biofilm production.
J Clin Pathol. 2020 Mar 25. Epub 2020 Mar 25. PMID: 32213553
AIMS: () is a life-threatening pathogen with high morbidity and mortality rates which causes nosocomial and community-acquired infections. Biofilm, considered to be a common virulence factor for pathogens, plays a significant role in recurrent and untreatable infections. Biofilm formation ofis mediated by synthesis of either poly-N-acetylglucosamine in an ica-dependent manner or surface proteins in an ica-independent manner. In some cases treatment is impossible and recurrent. In this study,-dependent biofilm-producingisolates were detected and the anti-biofilm effect of ascorbic acid against biofilm formation of isolates was investigated.
METHODS: A total of 21 methicillin-sensitive(MSSA) clinical isolates stored in our bacterial stock were used to detect-dependent biofilm-producing MSSA isolates. The anti-biofilm study was undertaken with three-dependent biofilm-producing isolates (MSSA2-4) and ATCC 29213 (MSSA1). Biofilms and the anti-biofilm effect of ascorbic acid were detected using the microtitre plate (MtP) method. 16S-rRNA,A andD genes and expression levels ofA andD of isolates were detected by RT-PCR.
RESULTS: The minimum inhibitory concentrations (MICs) of ascorbic acid prevented biofilm formation of MSSA1 and MSSA3. Also, 1/2 MIC of ascorbic acid prevented biofilm formation of MSSA3. It was observed that biofilm formation decreased with increased concentration. There was no significant increase ingene expression of MSSA1 and MSSA2. Expression ofA andD of MSSA3 decreased 13% and 38%, respectively. Expression ofA in MSSA4 decreased 12%.
CONCLUSION: The results of our study show that ascorbic acid can be used as an anti-biofilm agent to prevent biofilm formation ofand thus biofilm-related infections.