Abstract Title:

Ascorbic acid protects male rat brain from oral potassium dichromate-induced oxdative DNA damage and apoptotic changes: the expression patterns of caspase-3, P 53, Bax, and Bcl-2 genes.

Abstract Source:

Environ Sci Pollut Res Int. 2018 Feb 26. Epub 2018 Feb 26. PMID: 29484617

Abstract Author(s):

Ehsan H Abu Zeid, Mohamed M A Hussein, Haytham Ali

Article Affiliation:

Ehsan H Abu Zeid


Our study designed to study the potential of potassium dichromate (KCrO) oral exposure to induce damage in male rat brain and to compare the possible protective role of vitamin C (VC) either pre and/or concurrent supply against (KCrO) induced changes. Thirty male rats were divided into five groups. First control group received distilled water (C), second received 120 mg/kg b.wt (VC), third received 25 mg/kg b.wt KCrO(Cr), fourth group received VC together with KCrOby the same former doses (VC + Cr), and the fifth group received the same oral doses of VC 2 weeks prior to and along with KCrOfor 6 weeks (VC + Cr pro/co treated). The obtained results revealed that KCrOinduced a significant decrease in cholinergic activity, glutathione reductase GR activity, reduced glutathione content GSH and ATP levels. Furthermore, KCrOinduced a significant increase in oxidative DNA damage indicated by 8-hydroxy 2'-deoxyguanosine (8OH2'dG) and formation of apoptotic DNA ladders, significant increase in malondialdehyde (MDA), protein carbonyl, and lactate dehydrogenase enzyme. Increased mRNA expression of pro-apoptotic genes, including caspase-3, p53, and Bax, unlike Bcl-2 expression, was decreased. KCrOincreased caspase-3 and decreased Bcl-2 immuno-labeling. VC supply noticeably ameliorates KCrO-induced changes which were more significantly in VC pro and concurrent supplement rather than VC concurrent supply only. Finally, it is concluded that KCrOoral administration induced oxidative apoptotic changes in rat brain and confirms the usefulness of VC pre and concurrent supply for the amelioration of KCrO-induced events more significantly than VC concurrent supply only.

Study Type : Animal Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.