Asiatic acid inhibits OVX-induced osteoporosis and osteoclastogenesis. - GreenMedInfo Summary
Asiatic Acid Inhibits OVX-Induced Osteoporosis and OsteoclastogenesisRegulating RANKL-Mediated NF-κb and Nfatc1 Signaling Pathways.
Front Pharmacol. 2020 ;11:331. Epub 2020 Mar 27. PMID: 32292339
is a triterpenoid compound extracted from a medicinal plant. It has been used as a highly efficient compound for the treatment of cancer and hyperlipidemia, as well as possessing potential antiinflammatory properties. However, its effects on bone metabolism and osteoporosis haven't been reported. The purpose of our research were to reveal the biomolecular effects ofon osteoclasts, and its underlying molecular mechanisms regulating its effects on receptor activator of NF-κB ligand (RANKL)-induced signaling pathways. We found thatinhibited multinucleated tartrate-resistant acid phosphatase (TRAcP)-positive osteoclast differentiation and osteoclast induced bone loss. Real time PCR showed thatreduced the expression of down-cascade target genes including,,, and. Western blot and luciferase reporter gene assays revealed thatinhibits RANKL mediated NF-κB and NFATc1 signalings. Further,study demonstratedattenuates estrogen deficiency-induced bone loss in ovariectomized mice. MicroCT and histology analyses revealed that osteoclast numbers were significantly suppressed intreated groups. Furthermore, serum levels of TRAcP and CTX-1 were downregulated in treated groups. Taken together, our data show thatcan inhibit osteoclastic formation and reduce OVX-induced bone resorption through RANKL-activated NF-κB or NFATc1 signaling, suggesting thatmay be a potential and effective natural compound for the therapy of excessive RANKL-related osteolytic diseases.