n/a
Abstract Title:

[AstragalosideⅣ's Therapeutic Effect on Myocardial Infarction via Affecting Autophagy and the Mechanism Study].

Abstract Source:

Sichuan Da Xue Xue Bao Yi Xue Ban. 2021 Mar ;52(2):222-228. PMID: 33829695

Abstract Author(s):

Bin Zhang, Qian Ma, Fu-Zhen Ma, Jing Wang, Xu-Dong Liu, Ru-Hua He

Article Affiliation:

Bin Zhang

Abstract:

Objective: The purpose of this study was to investigate the protective effect of astragalosideⅣ (AS-Ⅳ) on neonatal rats' hypoxic/reoxygenated (H/R) injured myocardial cells and to explore its underlying mechanism.

Methods: Cardiac cells were extracted from newborn rats and divided into control, H/R, H/R-low AS-Ⅳ (0.1 μmol/L AS-Ⅳ), H/R-medium AS-Ⅳ (1 μmol/L AS-Ⅳ), H/R-high AS-Ⅳ (10 μmol/L AS-Ⅳ) and H/R-high AS-Ⅳ-AKT (10 μmol/L AS-Ⅳ+5 μmol/L AKT) groups. After 48 h of treatment, the contents of LC3-Ⅱ, p62, AKT, pAKT, rapamycin (mTOR) mammalian targets and uncoordinated 51-like kinase1 (ULK1) in cardiac myocytes were compared. Immunofluorescence staining was used to detect the expression of P62 in myocardium autophagosome.

Restults: AS-Ⅳ improved the proliferative activity of cardio AS-Ⅳ improved the proliferative activity of cardiomyocytes in H/R injury in a dose-dependent manner and inhibited the level of cell autophagy. However, when AKT inhibitors were added, the effect of AS-Ⅳ was partially inhibited (<0.05). Gene and protein expression showed that AS-Ⅳ had no significant effect on the expression of AKT and mTOR genes (>0.05), but could significantly promote the phosphorylation of AKT and mTOR (<0.05). Immunofluorescence staining results showed that high concentrations of the AS -Ⅳ can reverse H/R injury induced the expression of autophagy body P62.

Conclusion: AS-Ⅳ showed protection effect on H/R injured myocardial cells. The possible mechanism is by reducing the autophagy level via activating the mTOR signal in the PI3K/AKT pathway, thereby preventing H/R damage in neonatal rat cardiomyocytes.

Study Type : In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.