Induces Protective Colonic PD-L1 and Foxp3 Regulatory T Cells in an Acute Murine Experimental Model of Inflammatory Bowel Disease.
Gut Liver. 2019 Mar 15. Epub 2019 Mar 15. PMID: 30600673
Background/Aims: The current study aims to investigate the protective effects ofon the abnormal immune response to inflammatory bowel disease (IBD) in dextran sodium sulfate (DSS)-induced colitis.
Methods: Eight-week-old BALB/c mice were separated into five groups at random (control, DSS, DSS+B9 [1×10CFU], DSS+B8 [1×10CFU], and DSS+B7 [1×10CFU]). Colitis was induced by 5% DSSfor 7 days, at which time we assessed weight, the disease activity index (DAI) score, and the histological damage score. The nuclear transcription factor Foxp3 (a marker of Treg cells), cytokines interleukin-10 (IL-10) and transforming growth factorβ1 (TGF-β1), and related proteins PD-L1 and PD-1 were detected by an immunohistochemical method and Western blot.
Results: increased weight, decreased DAI scores and histological damage scores, increased the protein expression of Foxp3 (p＜0.05) and cytokines IL-10 and TGF-β1 in mouse colon tissue (p＜0.05), and increased the expression of PD-L1 in the treatment groups relative to that in the DSS group (p＜0.05). The effect ofon Foxp3 and PD-L1 was dose dependent in the treatment groups (p＜0.05). PD-L1 was positively correlated with Foxp3, IL-10, and TGF-β1.
Conclusions: In a mouse model of IBD,can alleviate intestinal epithelial injury and maintain intestinal immune tolerance and thus may have potential therapeutic value for the treatment of immune damage in IBD.