Bioactive Compounds ofEssential Oil as Antibacterial Agents againstD.
Microorganisms. 2019 Sep 19 ;7(9). Epub 2019 Sep 19. PMID: 31546941
Urogenital tract infection caused by obligate intracellular bacteriumD (D) is a leading cause of sexually transmitted diseases. Essential oil (EO) ofhas a broad antimicrobial spectrum. The aim of this study was to evaluate the antimicrobial activity of the bioactive compounds (p-cymene, thymoquinone, carvacrol, and thymol) ofEO againstD. The cytotoxic effects of the compounds were determined by MTT assay. In order to quantify the anti-chlamydial activity of the compounds, HeLa cells were infected withD orD treated previously with the compounds. The titer of the infectiousD was determined by indirect immunofluorescence assay. The minimum inhibitory concentrations of the compounds were evaluated by direct quantitative PCR. None of the compounds showed a cytotoxic effect on HeLa cells in the concentrations tested. According to the immunofluorescence assay, all of the compounds significantly inhibited the growth ofD. The quantitative PCR revealed that the minimum concentration that exerted anti-chlamydial activity was 3.12µM in the case of thymoquinone and p-cymene, while that of carvacrol and thymol was 6.25 µM. Therefore, it can be concluded that bioactive compounds ofEO could be used as effective antimicrobial agents againstD.