Beneficial role of biochanin A on cutaneous and renal tissues of ovariectomized rats treated with anastrozole.
Life Sci. 2018 May 15 ;201:9-16. Epub 2018 Mar 19. PMID: 29567078
Azza A A Galal
AIMS: This study was designed to assess the beneficial role of biochanin A (BCA) in the protection of the cutaneous and renal tissues of ovariectomized rats, even in those treated with anastrozole (ANA).
MATERIALS AND METHODS: For this purpose, 60 adult female Wistar rats were allocated into 6 equal groups. Rats in group I (sham) underwent a sham operation and received distilled water orally. In groups II and III, OVX rats received either distilled water orally or DMSO intraperitoneally, respectively. In groups IV and V, OVX rats were either treated orally with 0.5 mg ANA/kg.b.wt. or administered 5 mg of BCA/kg.b.wt. intraperitoneally, respectively. In group VI (OVX-ANA-BCA), OVX rats were co-treated with BCA and ANA. All treatments were given daily for 20 weeks.
KEY FINDINGS: The IP administration of BCA significantly decreased serum levels of urea, creatinine, uric acid and MDA and significantly increased serum CAT, GSH and TAC and cutaneous IL-4 and GATA3 concentrations in the fifth and sixth groups compared to OVX-DMSO and OVX-ANA groups, respectively. Additionally, it induced down-regulation of renal TNF-α and iNOS expression as well as up-regulation of cutaneous TGF-β expression. Furthermore, BCA ameliorated to variable degrees histological changes of renal and cutaneous tissue related to ovariectomy and/or ANA treatment.
SIGNIFICANCE: These data suggest that BCA might be a useful alternative estrogen therapy for the management of renal and cutaneous changes observed in postmenopausal women, even in those treated with ANA.