Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

Article Publish Status: FREE
Abstract Title:

Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury.

Abstract Source:

World J Gastroenterol. 2015 Sep 21 ;21(35):10091-103. PMID: 26401074

Abstract Author(s):

Kazutaka Yoshida, Yusuke Ushida, Tomoko Ishijima, Hiroyuki Suganuma, Takahiro Inakuma, Nobuhiro Yajima, Keiko Abe, Yuji Nakai

Article Affiliation:

Kazutaka Yoshida

Abstract:

AIM: To investigate the effects of broccoli sprout extract (BSEx) on liver gene expression and acute liver injury in the rat.

METHODS: First, the effects of BSEx on liver gene expression were examined. Male rats were divided into two groups. The Control group was fed the AIN-76 diet, and the BSEx group was fed the AIN-76 diet containing BSEx. After a 10-d feeding period, rats were sacrificed and their livers were used for DNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) analyses. Next, the effects of BSEx on acute liver injury were examined. In experiments using acute liver injury models, 1000 mg/kg acetaminophen (APAP) or 350 mg/kg D-galactosamine (D-GalN) was used to induce injury. These male rats were divided into four groups: Control, BSEx, Inducer (APAP or D-GalN), and Inducer+BSEx. The feeding regimens were identical for the two analyses. Twenty-four hours following APAP administration via p.o. or D-GalN administration via i.p., rats were sacrificed to determine serum aspartate transaminase (AST) and alanine transaminase (ALT) levels, hepatic glutathione (GSH) and thiobarbituric acid-reactive substances accumulation and glutathione-S-transferase (GST) activity.

RESULTS: Microarray and real-time RT-PCR analyses revealed that BSEx upregulated the expression of genes related to detoxification and glutathione synthesis in normal rat liver. The levels of AST (70.91± 15.74 IU/mL vs 5614.41 ± 1997.83 IU/mL, P<0.05) and ALT (11.78± 2.08 IU/mL vs 1297.71 ± 447.33 IU/mL, P<0.05) were significantly suppressed in the APAP + BSEx group compared with the APAP group. The level of GSH (2.61± 0.75 nmol/g tissue vs 1.66 ± 0.59 nmol/g tissue, P<0.05) and liver GST activity (93.19± 16.55 U/g tissue vs 51.90 ± 16.85 U/g tissue, P<0.05) were significantly increased in the APAP + BSEx group compared with the APAP group. AST (4820.05± 3094.93 IU/mL vs 12465.63 ± 3223.97 IU/mL, P<0.05) and ALT (1808.95± 1014.04 IU/mL vs 3936.46 ± 777.52 IU/mL, P<0.05) levels were significantly suppressed in the D-GalN + BSEx group compared with the D-GalN group, but the levels of AST and ALT in the D-GalN + BSEx group were higher than those in the APAP + BSEx group. The level of GST activity was significantly increased in the D-GalN + BSEx group compared with the D-GalN group (98.04± 15.75 U/g tissue vs 53.15 ± 8.14 U/g tissue, P<0.05).

CONCLUSION: We demonstrated that BSEx protected the liver from various types of xenobiotic substances through induction of detoxification enzymes and glutathione synthesis.

Print Options


Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2019 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.