Broccoli sprouts juice prevents lens protein aggregation in streptozotocin-induced diabetic rat.
Int J Ophthalmol. 2019 ;12(9):1380-1385. Epub 2019 Sep 18. PMID: 31544030
AIM: To investigate the effect of broccoli sprouts juice in preventing lens protein aggregation in diabetic rat model.
METHODS: Totally 25 male Wistar rats were divided into negative control group, diabetic group without juice treatment as positive control and diabetic group given broccoli sprouts juice 0.25, 0.5 and 1 g/d for 28d. Diabetic rat model was obtained by administering a single dose streptozotocin 65 mg/kg intraperitoneal. At the end of the study, all rats were examined for fasting blood sugar level (FBS), visual cataract score using slit lamp biomicroscope and lensαB-crystallin expression using Western blot method. Statistical analysis was performed using one way ANOVA with post hoc test, correlation test, and simple linear regression.
RESULTS: Positive control group had the highest cataract score and lens aggregatedαB-crystallin expression. Broccoli sprout juice dose of 1 g/d group had the mildest cataract score, as well as the expression of lens aggregated αB-crystallin compared to treatments groups 1 and 2, opposite to lens native αB-crystallin expression. The broccoli sprout juice groups gave a significant decrease in cataract score, and also in lens aggregated αB-crystallin expression in diabetic rat models (<0.05).
CONCLUSION: Broccoli sprout juice has a significant effect in preventing lens protein aggregation in diabetic rat model. The higher dose gives better visual cataract scores, lower lens aggregatedαB-crystallin expression and higher lens native αB-crystallin expression.