Evaluation of antioxidant and anti-inflammatory efficacy of caffeine in rat model of neurotoxicity.
Nutr Neurosci. 2018 Mar 7:1-8. Epub 2018 Mar 7. PMID: 29514562
Eman N Hosny
OBJECTIVE: The present study aims to investigate the neuroprotective effect of caffeine against aluminum chloride (AlCl)-induced neurotoxicity in rats.
METHODS: Twenty-one male albino rats were divided into 3 groups: control, AlCl-intoxicated group that received daily oral administration of AlCl(100 mg/kg for 30 days) and protected group injected daily with caffeine (20 mg/kg intraperitoneally) one hour before oral administration of AlClfor 30 days. Levels of lipid peroxidation, reduced glutathione, and nitric oxide and the activities of acetylcholinesterase (AchE) and Na/K-ATPase were measured spectrophotometrically. Tumor necrosis factor-α (TNF-α) was evaluated by ELISA kit.
RESULTS: The data revealed evidence of oxidative and nitrosative stress in the cerebral cortex, hippocampus, and striatum of AlCl-intoxicated rats. This was indicated from the increased levels of lipid peroxidation and nitric oxide together with the decreased level of reduced glutathione. Moreover, the daily AlCladministration increased AchE and Na/K-ATPase activities and the level of TNF-α in the selected brain regions. Protection with caffeine ameliorated the oxidative stress induced by AlClin the cerebral cortex, hippocampus, and striatum. In addition, caffeine restored the elevated level of TNF-α in the hippocampus and striatum. This was accompanied by an improvement in the activities of AchE and Na/K-ATPase in the studied brain regions.
DISCUSSION AND CONCLUSIONS: The present findings clearly indicate that caffeine provides a significant neuroprotection against AlCl-induced neurotoxicity mediated by its antioxidant, anti-inflammatory, and anticholinesterase properties.