Carnosic Acid Impedes Cell Growth and Enhances Anti-cancer Effects of Carmustine and Lomustine in Melanoma.
Biosci Rep. 2018 May 22. Epub 2018 May 22. PMID: 29789400
Carnosic acid (CA), a major polyphenolic diterpene present in, has been reported to have multiple functions, including anti-tumor activity.The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU).This study finds that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also find that CA triggers cell cycle arrest at G0/G1 phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we find that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT)This study concludes that CA may be safe and useful as a novel chemotherapeutic agent.