β-carotene Inhibits Expression of c-Myc and Cyclin E in-infected Gastric Epithelial Cells.
J Cancer Prev. 2019 Sep ;24(3):192-196. Epub 2019 Sep 30. PMID: 31624725
Background: infection is a major risk factor in the development of gastric cancer.infection of gastric epithelial cells increases the levels of reactive oxygen species (ROS), activates oncogenes, and leads toβ-catenin-mediated hyper-proliferation. β-Carotene reduces ROS levels, inhibits oxidant-mediated activation of inflammatory signaling and exhibits anticancer properties. The present study was carried out to determine if β-carotene inhibits-induced cell proliferation and the expression of oncogenes c-myc and cyclin E by reducing the levels ofβ-catenin and phosphorylated glycogen synthase kinase 3β (p-GSK3β).
Methods: Gastric epithelial AGS cells were pre-treated withβ-carotene (5 and 10 μM) for 2 hours prior toinfection and cultured for 6 hours (for determination of the levels of p-GSK3β, GSK3β, and β-catenin) and 24 hours (for determination of cell viability and protein levels of c-myc and cyclin E). Cell viability was determined by the MTT assay and protein levels were determined via western blot-based analysis.
Results: β-Carotene inhibited-induced increases in the percentage of viable cells, phosphorylated GSK3β (p-GSK3β), and the levels of β-catenin, c-myc and cyclin E.
Conclusions: β-Carotene inhibits-induced hyper-proliferation of gastric epithelial cells by suppressingβ-catenin signaling and oncogene expression.