Catechin isolated from faba beans (L.): insights from oxidative stress and hypoglycemic effect in yeast cells through confocal microscopy, flow cytometry, andstrategy.
J Biomol Struct Dyn. 2021 Jun 30:1-11. Epub 2021 Jun 30. PMID: 34192480
Dhiraj Kumar Choudhary
The present aim of this investigation was to evaluate the effect of catechin from faba beans on oxidative stress and glucose uptake in yeast cells. Flow cytometry approach indicated that 2-NBDG (1.98 ± 0.37) seed extract had a lower relative fluorescence signal than methanol (5.98 ± 0.67) and acetone seed extract (4.43 ± 0.55). In comparison to the control and seed extract, HOexposure increased the apoptosis rate of yeast cells from 8.20% to 64.80%. Yeast cells incubated with HOproduced significantly more ROS intensity (162 ± 4.32, < 0.05) than control cells (118 ± 2.52, < 0.05) and less than seed extract-treated cells. Molecular dynamics simulation studies were performed for cat:α-amylase (catechin-α-amylase complex) which revealed the stable and mixed mode of inhibition during a simulation. The synergistic action of polyphenols or catechin present in seed extract may be responsible for the anti-oxidative stress and hypoglycaemic effects. The findings of this study may provide insight into the further development of a novel antidiabetic drug for T2DM. Communicated by Ramaswamy H. Sarma.