Abstract Title:

Celery Seed Extract Blocks Peroxide Injury in Macrophages via Notch1/NF-κB Pathway.

Abstract Source:

Am J Chin Med. 2015 ;43(3):443-55. Epub 2015 Apr 27. PMID: 25916469

Abstract Author(s):

Yanhong Si, Shoudong Guo, Yongqi Fang, Shucun Qin, Furong Li, Ying Zhang, Peng Jiao, Chunduo Zhang, Linlin Gao

Article Affiliation:

Yanhong Si

Abstract:

Oxidized low-density lipoprotein (ox-LDL)-induced macrophage foam cell formation and injury is one of the major atherogenic factors. This study is aimed to investigate the protective effect of celery seed extract (CSE) on ox-LDL-induced injury of macrophages and the underlying signaling pathway. RAW264.7 macrophages were pre-incubated with CSE for 24 h, followed by stimulation with ox-LDL. Oil red O staining and enzymatic colorimetry indicated CSE significantly lessened lipid droplets and total cholesterol (TC) content in ox-LDL-injured macrophages. ELISA revealed that CSE decreased the secretion of inflammatory cytokine TNF-α and IL-6 by 12-27% and 5-15% respectively. MTT assay showed CSE promoted cell viability by 16-40%. Cell apoptosis was also analyzed by flow cytometry and laser scanning confocal microscope and the data indicated CSE inhibited ox-LDL-induced apoptosis of macrophages. Meanwhile, western blot analysis showed CSE suppressed NF-κBp65 and notch1 protein expressions stimulated by ox-LDL in macrophages. These results suggest that CSE inhibits ox-LDL-induced macrophages injury via notch1/NF-κB pathway.

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