n/a
Article Publish Status: FREE
Abstract Title:

Characterization of the Anti-Cancer Activity of the Probiotic BacteriumUsing 2D vs. 3D Culture in Colorectal Cancer Cells.

Abstract Source:

Biomolecules. 2019 Oct 1 ;9(10). Epub 2019 Oct 1. PMID: 31581581

Abstract Author(s):

Joo-Eun Lee, Jina Lee, Ji Hyun Kim, Namki Cho, Sung Hoon Lee, Sung Bum Park, Byumseok Koh, Dukjin Kang, Seil Kim, Hee Min Yoo

Article Affiliation:

Joo-Eun Lee

Abstract:

The aim of this study was to investigate the potential anti-cancer effects of probiotic cell-free supernatant (CFS) treatment usingfor colorectal cancer (CRC) in 3D culture systems. Cell viability was assessed using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2-tetrazolium, inner salt) assays, whereas apoptosis was monitored through RT-qPCR analysis of Bax, Bak, Noxa, and Bid mRNA expressions in addition to flow cytometry analysis ofcell-free supernatant (LCFS) treatment. Our results showed that the anti-cancer effect of LCFS on cell viability was pronouncedly enhanced in 3D-cultured HCT-116 cells, which was linked to the increased level of cleaved caspase 3. Additionally, upregulation of apoptotic marker gene mRNA transcription was dramatically increased in 3D cultured cells compared to 2D systems. In conclusion, this study suggests that LCFS enhances the activation of intrinsic apoptosis in HCT-116 cells and the potential anti-cancer effects of Lactobacilli mixtures in 3D culture systems. All in all, our study highlights the benefits of 3D culture models over 2D culture modeling in studying the anti-cancer effects of probiotics.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.