Protective effects of chebulic acid fromRetz. against-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells.
Food Sci Biotechnol. 2019 Apr ;28(2):555-562. Epub 2018 Sep 27. PMID: 30956868
Although chebulic acid isolated fromhas diverse biological effects, its effects on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of downstream genes have not been elucidated. The purpose of this research is to investigate the hepatoprotective mechanism of chebulic acid against oxidative stress produced by-butyl hydroperoxide (-BHP) in liver cells. The treatment with chebulic acid attenuated cell death in-BHP-induced HepG2 liver cells and increased intracellular glutathione content, upregulated the activity of heme oxygenase-1, and also increased the translocation of Nrf2 into the nucleus and Nrf2 target gene expression in a dose-dependent manner. The exposure of chebulic acid activated the phosphorylation of mitogen-activated protein kinases. The overall result is that chebulic acid has cytoprotective effect on-BHP-induced hepatotoxicity in HepG2 cells through Nrf2-mediated antioxidant enzymes.