Chemical characteristics, antioxidant capacities and hepatoprotection of polysaccharides from pomegranate peel.
Carbohydr Polym. 2018 Dec 15 ;202:461-469. Epub 2018 Sep 7. PMID: 30287023
This proposed work aimed to investigate the chemical characteristic, antioxidant capacities and hepatoprotection effect of pomegranate peel polysaccharides (PPP) on CCl-induced oxidative damage in mice. PPP was identified as the acidic heteropolysaccharides by HPLC methods. In vitro test showed that PPP had excellent reducing power and scavenging effects against free radicals. Administration of PPP (50, 100 and 200 mg/kg·bw) in mice before the injection of CClcould observably antagonize the increased serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and hepatic malonaldehyde level in CCl-induced mice, especially administrated with 200 mg/kg·bw of PPP. Hepatic enzymatic activities of total superoxide dismutase, glutathione peroxidase, catalase and non-enzymatic activity of glutathione were markedly increased at high dosage of PPP, respectively. In addition, histopathological observation of liver further proved these biochemical characteristics. Therefore, it can be concluded that PPP exhibits strong protective effects against CCl-induced liver injury in mice.