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Article Publish Status: FREE
Abstract Title:

Chemoprotective Effects of Xanthohumol against the Carcinogenic Mycotoxin Aflatoxin B1.

Abstract Source:

Foods. 2021 Jun 9 ;10(6). Epub 2021 Jun 9. PMID: 34207931

Abstract Author(s):

Alja Štern, Veronika Furlan, Matjaž Novak, Martina Štampar, Zala Kolenc, Katarina Kores, Metka Filipič, Urban Bren, Bojana Žegura

Article Affiliation:

Alja Štern

Abstract:

The present study addresses the chemoprotective effects of xanthohumol (XN), a prenylated flavonoid found in the female inflorescences (hops) of the plantL., against the carcinogenic food contaminant aflatoxin B1 (AFB1). The chemical reactions of XN and its derivatives (isoxanthohumol (IXN), 8-prenylnaringenin (8-PN), and 6-prenylnaringenin (6-PN)) with the AFB1 metabolite, aflatoxin B1 exo-8,9-epoxide (AFBO), were investigated, by calculating activation free energies (ΔG) at the Hartree-Fock level of theory in combination with the 6-311++G(d,p) basis set and two implicit solvation models. The chemoprotective effects of XN were investigatedin the metabolically competent HepG2 cell line, analyzing its influence on AFB1-induced cytotoxicity using the MTS assay, genotoxicity using the comet andγH2AX assays, and cell cycle modulation using flow cytometry. Our results show that the ΔGrequired for the reactions of XN and its derivatives with AFBO are comparable to theΔGrequired for the reaction of AFBO with guanine, indicating that XN, IXN, 8-PN, and 6-PN could act as scavengers of AFBO, preventing DNA adduct formation and DNA damage induction. This was also reflected in the results from theexperiments, where a reduction in AFB1-induced cytotoxicity and DNA single-strand and double-strand breaks was observed in cells exposed to combinations of AFB1 and XN, highlighting the chemoprotective effects of this phytochemical.

Study Type : In Vitro Study

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