Article Publish Status: FREE
Abstract Title:

Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-β1/Smad7 Signaling Pathwayand.

Abstract Source:

Front Pharmacol. 2017 ;8:929. Epub 2017 Dec 19. PMID: 29311932

Abstract Author(s):

Fan Yang, Lei Luo, Zhi-De Zhu, Xuan Zhou, Yao Wang, Juan Xue, Juan Zhang, Xin Cai, Zhi-Lin Chen, Qian Ma, Yun-Fei Chen, Yu-Jie Wang, Ying-Ying Luo, Pan Liu, Lei Zhao

Article Affiliation:

Fan Yang


Chlorogenic acid (CGA) is a phenolic acid that has a wide range of pharmacological effects. However, the protective effects and mechanisms of CGA on liver fibrosis are not clear. This study explored the effects of CGA on miR-21-regulated TGF-β1/Smad7 liver fibrosis in the hepatic stellate LX2 cell line and in CCl4-induced liver fibrosis in Sprague-Dawley rats.The mRNA expression of miR-21, Smad7, connective tissue growth factor (CTGF),α-smooth muscle actin (α-SMA), tissue inhibitor of metalloproteinase 1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), and transforming growth factor-β1 (TGF-β1) and the protein levels of Smad2, p-Smad2, Smad3, p-Smad3, Smad2/3, p-Smad2/3, Smad7, CTGF, α-SMA, TIMP-1, MMP-9 and TGF-β1 were assayed in LX2 cells and liver tissue. The effects of CGA after miR-21 knockdown or overexpression were analyzed in LX2 cells. The liver tissue and serum were collected for histopathological examination, immunohistochemistry (IHC) and ELISA.The mRNA expression of miR-21, CTGF,α-SMA, TIMP-1, and TGF-β1 and the protein expression of p-Smad2, p-Smad3, p-Smad2/3, CTGF, α-SMA, TIMP-1, and TGF-β1 were inhibited by CGA bothand. Meanwhile, CGA elevated the mRNA and protein expression of Smad7 and MMP-9. After miR-21 knockdown and overexpression, the downstream molecules also changed accordingly. CGA also lessened the degree of liver fibrosis in the pathological manifestation and reducedα-SMA and collagen I expression in liver tissue and TGF-β1 in serum.CGA might relieve liver fibrosis through the miR-21-regulated TGF-β1/Smad7 signaling pathway, which suggests that CGA might be a new anti-fibrosis agent that improves liver fibrosis.

Study Type : Animal Study, In Vitro Study

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