Abstract Title:

Chronic E-cigarette Exposure Alters the Human Bronchial Epithelial Proteome.

Abstract Source:

Am J Respir Crit Care Med. 2018 Feb 26. Epub 2018 Feb 26. PMID: 29481290

Abstract Author(s):

Arunava Ghosh, Raymond C Coakley, Teresa Mascenik, Temperance R Rowell, Eric S Davis, Keith Rogers, Megan J Webster, Hong Dang, Laura E Herring, M Flori Sassano, Alessandra Livraghi-Butrico, Scott K Van Buren, Lee M Graves, Melissa A Herman, Scott H Randell, Neil E Alexis, Robert Tarran

Article Affiliation:

Arunava Ghosh


RATIONAL: E-cigarettes vaporize propylene glycol/vegetable glycerin (PG/VG), nicotine and flavorings. However, the long-term health effects of exposing lungs to vaped e-liquids are unknown.

OBJECTIVES: To determine the effects of chronic vaping on pulmonary epithelia.

METHODS: We performed research bronchoscopies on healthy non-smokers, cigarette smokers and e-cigarettes users (vapers) and obtained bronchial brush biopsies and lavage samples from these subjects for proteomic investigation. We further employed in vitro and murine exposure models to support our human findings.

MEASUREMENTS AND MAIN RESULTS: Visual inspection by bronchoscopy revealed that vaper's airways appeared friable and erythematous. Epithelial cells from biopsy samples revealed ~300 proteins that were differentially expressed in smokers and vaper's airways, with only 78 proteins being commonly altered in both groups and 113 uniquely altered in vapers. For example, CYP1B1, MUC5AC and MUC4 levels were increased in vapers. Aerosolized PG/VG alone significantly increased MUC5AC protein in human airway epithelial cultures and in murine nasal epithelia in vivo. We also found that e-liquids rapidly entered cells and that PG/VG reduced membrane fluidity and impaired protein diffusion.

CONCLUSIONS: We conclude that chronic vaping exerts marked biological effects on the lung and that these effects may in part be mediated by the PG/VG base. These changes are likely not harmless and may have clinical implications for the development of chronic lung disease. Further studies will be required to determine the full extent of vaping on the lung.

Study Type : Animal Study, Human Study, In Vitro Study
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