Anticancer Effects ofJ. Presl, Cinnamaldehyde, 4 Hydroxycinnamic Acid and Eugenol on an Oral Squamous Cell Carcinoma Cell Line.
J Contemp Dent Pract. 2020 Sep 1 ;21(9):1027-1033. Epub 2020 Sep 1. PMID: 33568591
AIM AND OBJECTIVE: The present study was conducted to assess theanticancer effects ofJ. Presl extract and its active constituents, such as cinnamaldehyde, 4 hydroxycinnamic acid, and eugenol on oral squamous cell carcinoma cell line.
MATERIALS AND METHODS: Aqueous, ethanolic, and hydroalcoholic extracts ofJ. Presl (bark) were prepared using standardized protocols. Cinnamaldehyde, 4 hydroxycinnamic acid, and eugenol were quantified in the extracts. Total saponins, tannins, and polyphenols were quantified in the selected extracts. A commercially available SCC-25 cell line was cultured according to standard protocol. The anticancer effects of the extract, active compounds, and standard cisplatin were assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxicity, acridine orange/ethidium bromide staining, DNA, fragmentation assay, cell cycle analysis by flow cytometry, and JC-1 staining (5,5',6,6'-tetrachloro1,1',3,3'tetraethylbenzimidazolylcarbocyanine iodide).
RESULTS: The hydroalcoholic extracts demonstrated a higher quantity of the active ingredients cinnamaldehyde, 4 hydroxycinnamic acid, and eugenol. The selected extract and active compounds demonstrated anticancer effects via apoptosis induction and S-phase arrest. Apoptosis induction was exerted by the extract via alteration in mitochondrial membrane potential.
CONCLUSION: J. Presl and its active compounds exhibitedanticancer effects on oral squamous cell carcinoma. Further studies in animal models have to be carried out to assess toxicity andeffects.
CLINICAL SIGNIFICANCE: The anticancer properties ofJ. Presl could be explored further for prevention and management of oral squamous cell carcinoma.