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Abstract Title:

Synergistic Effect of Apigenin and Curcumin on Apoptosis, Paraptosis and Autophagy-related Cell Death in HeLa Cells.

Abstract Source:

Anticancer Res. 2021 Mar ;41(3):1271-1282. PMID: 33788718

Abstract Author(s):

Sera Kayacan, Kaan Yilancioglu, Ayse Seda Akdemir, Fatma Kaya-Dagistanli, Gulay Melikoglu, Melek Ozturk

Article Affiliation:

Sera Kayacan

Abstract:

BACKGROUND/AIM: We aimed to investigate the synergistic effects of apigenin and curcumin on the cross-talk between apoptosis and autophagic cell death, as well as on paraptosis in HeLa cells.

MATERIALS AND METHODS: Cell viability was measured using the MTT assay. Synergistic effects were measured using the Bliss independence model. qRT-PCR was used to study the expression of genes related to apoptosis, autophagic cell death, and cross-talk. GRP78/BiP immunostaining was used to identify endoplasmic reticulum (ER) stress.

RESULTS: Treatment with a combination of apigenin and curcumin increased the expression levels of genes related to cell death in HeLa cells 1.29- to 27.6-fold. The combination of curcumin and apigenin showed a synergistic anti-tumor effect via cross-talk between processes leading to apoptosis and autophagic cell death, as well as ER stress-associated paraptosis. GRP78 expression was down-regulated, and massive cytoplasmic vacuolization was observed in HeLa cells.

CONCLUSION: The combination of curcumin and apigenin is an effective potential therapeutic for cervical cancers.

Study Type : In Vitro Study

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Sayer Ji
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