Abstract Title:

Andrographolide and 14-deoxy-11,12-didehydroandrographolide from Andrographis paniculata attenuate high glucose-induced fibrosis and apoptosis in murine renal mesangeal cell lines.

Abstract Source:

J Ethnopharmacol. 2010 Aug 30. Epub 2010 Aug 30. PMID: 20813180

Abstract Author(s):

Meng-Jen Lee, Yerra Koteswara Rao, Keru Chen, Yi-Ching Lee, Yi-Shuan Chung, Yew-Min Tzeng

Article Affiliation:

Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Wufeng, Taiwan, ROC.


ETHNOPHARMACOLOGICAL RELEVANCE: Extracts of Andrographis paniculata Nees is used for various ethnomedical conditions including hyperglycemia and hypertension complications. AIM OF THE STUDY: The purpose of this study is to evaluate the anti-diabetic nephropathy effect of diterpene lactones andrographolide (AP1) and 14-deoxy-11,12-didehydroandrographolide (AP2) from A. paniculata. MATERIALS AND METHODS: MES-13, a SV40 transformed murine glomerular mesangial cell line, was cultured in high concentration of glucose to induce diabetic nephropathy phenotypes, which include secretion of extracellular matrix protein fibronectin, cytokine TGF-beta, states of oxidative stress, and apoptosis marker caspase-3. RESULTS: Our data suggest that addition of compounds AP1 or AP2 reduce the phenotypes indicating diabetic nephropathy in MES-13 cells. The compound AP2 showed potent activity than AP1 in the reduction of apoptosis marker caspase-3, fibrosis marker TGF-beta, and PAI-1. Furthermore, AP1 and AP2 do not have antioxidant ability in acellular environment, however addition of AP1 and AP2 reduced intracellular oxidative states in high glucose cultured MES-13 cells. CONCLUSION: This is the first report on anti-diabetic nephropathy effect of AP1 and AP2 in part due to regulation of intracellular signaling transduction, not mere clearance of reactive oxygen species. Thus, this study may useful for drug development or food supplement for diabetes and nephropathy from A. paniculata.

Study Type : Animal Study

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