Inhibitory Effect ofβ-Carotene on-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells.
Antioxidants (Basel). 2019 Dec 11 ;8(12). Epub 2019 Dec 11. PMID: 31835889
infection causes the hyper-proliferation of gastric epithelial cells that leads to the development of gastric cancer. Overexpression of tumor necrosis factor receptor associated factor (TRAF) is shown in gastric cancer cells. The dietary antioxidantβ-carotene has been shown to counter hyper-proliferation in-infected gastric epithelial cells. The present study was carried out to examine theβ-carotene mechanism of action. We first showed thatinfection decreases cellular IBα levels while increasing cell viability, NADPH oxidase activity, reactive oxygen species production, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) activation, and TRAF1 and TRAF2 gene expression, as well as protein-protein interaction in gastric epithelial AGS cells. We then demonstrated that pretreatment of cells with β-carotene significantly attenuates these effects. Our findings support the proposal that β-carotene has anti-cancer activity by reducing NADPH oxidase-mediated production of ROS, NF-B activation and NF-B-regulated TRAF1 and TRAF2 gene expression, andhyper-proliferation in AGS cells. We suggest that the consumption of β-carotene-enriched foods could decrease the incidence of H. pylori-associated gastric disorders.