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Abstract Title:

Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal Injury.

Abstract Source:

Evid Based Complement Alternat Med. 2020 ;2020:7202519. Epub 2020 Jan 13. PMID: 32419819

Abstract Author(s):

Yi Shan Huang, Xu Wang, Zhendong Feng, Hailan Cui, Zebing Zhu, Chenhui Xia, Xueting Han, Wei Jing Liu, Yu Ning Liu

Article Affiliation:

Yi Shan Huang

Abstract:

Hypertensive renal injury is a primary etiology of end-stage renal disease, and satisfactory therapeutic strategies are urgently required., a traditional Chinese herb, has potential renoprotective benefits and is widely used in the treatment of many kidney diseases. To investigate the mechanisms underlying the renoprotective effect ofon hypertensive renal injury, we studied the effect ofon tubular epithelial cells (TECs) in a spontaneously hypertensive rat (SHR) model and angiotensin II- (AngII-) cultured primary TECs. Our study showed thattreatment could decrease 24-hour urine albumin, albumin-to-creatinine ratio (ACR),2-MG level, and kidney injury molecule-1 (kim-1) level in SHR urine, alleviate interstitial fibrosis, and reduce-smooth muscle actin (-SMA) expression in SHR kidney. In primary TECs, medicated serum containing(CSM) might significantly reduce the AngII-induced production of kim-1 and neutrophil gelatinase-associated lipocalin (NGAL). Furthermore,treatment could decrease TEC apoptosis in SHRs as assessed by the terminal transferase-mediated biotin dUTP nick-end labeling (TUNEL) assay. CSM could inhibit caspase-3 activity and enhance cellular viability as measured by methyl thiazolyl tetrazolium in AngII-cultured TECs, suggesting that CSM might reduce the apoptosis level in TECs induced by AngII. We found that the SIRT1 expression level was markedly lowered, while the protein level of acetylated-p53 was elevated in the TECs of patients with hypertensive renal injury and SHRs.presented the effect of regulating the SIRT1/p53 pathway. Further SIRT1 inhibition with EX527 reversed the effect ofon AngII-induced apoptosis. Taken together, our results indicate thatoffers a protective effect on TECs under hypertensive conditions, which may be related to its antiapoptotic effect through regulation of the SIRT1/p53 pathway.

Study Type : Animal Study

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