Crocin reduces A. fumigatus-induced airway inflammation and NF-κB signal activation. - GreenMedInfo Summary
Crocin reduces A. fumigatus-induced airway inflammation and NF-κB signal activation.
J Cell Biochem. 2017 Aug 8. Epub 2017 Aug 8. PMID: 28786504
Chronic obstructive pulmonary disease (COPD) is a chronic airway inflammation and its exacerbation is often accompanied by A. Aspergillus infection. Increasing evidences demonstrated the potent antioxidant and anti-inflammatory effects of crocin. However, the role of crocin in A. Aspergillus-induced inflammation is still unknown. We aimed to evaluate the role of crocin in inflammation response induced by A. Aspergillus in human bronchial epithelial cells and the possible mechanisms. BEAS-2B and NHBE cells were pre-treated with crocin for 24 h, and then A. fumigatus conidia were added for 24 h. A. Aspergillus treatment exhibited a significant higher TNF-α, IL-8, IL-6 and IL-1β level (P < 0.05), whereas crocin pre-treatment significantly inhibited A. Aspergillus induced the pro-inflammatory cytokines (P < 0.05). NF-κB inhibitor PDTC inhibited pro-inflammatory cytokines release triggered by A. Aspergillus (P < 0.05). Furthermore, crocin suppressed A. Aspergillus induced NF-κB p65 nuclear translocation, the phosphorylation of IKKα and IκBα, the degradation of IκBα and NF-κB reporter activity. Crocin pre-treatment also resulted in an inhibition of A. Aspergillus-induced ROS production (P < 0.05). Taken together, these results indicate that crocin may prevents A. Aspergillus-induced inflammation through suppressing NF-κB signal pathway. This article is protected by copyright. All rights reserved.