The cucurbitacins D, E, and I from(L.) upregulate thegene and induce cell-cycle arrest in human gastric cancer cell line AGS.
Iran J Basic Med Sci. 2018 Mar ;21(3):253-259. PMID: 29511491
Objectives: Cucurbitacins exhibit a range of anti-cancer functions. We investigated the effects of cucurbitacins D, E, and I purified from(L.) A. Rich fruits on some apoptotic and autophagy genes in human gastric cancer cell line AGS.
Materials and Methods: Using quantitative reverse transcription PCR (qRT-PCR), the expression of,,,, andgenes were quantified in AGS cells 24 hr after treatment with cucurbitacins D, E, and I at concentrations 0.3, 0.1 and 0.5μg/ml, respectively. Cell cycle and death were analyzed by flflowcytometry.
Results: Purified cucurbitacins induced sub-G1 cell-cycle arrest and cell death in AGS cells and upregulatedmRNA effectively, but showed a very low effect on,, andmRNA levels. Also after treatment with cucurbitacin I at concentration 0.5μg/ml,mRNA levels were increased about 4.4 times. Pairwise comparison of the effect of cucurbitacins D, E, and I onmRNA expression showed that the cucurbitacin I effect is 1.3 and 1.1 times that of cucurbitacins E and D, respectively; cucurbitacin D effect is 1.2 times that of cucurbitacin E (<0.05).analysis showed that among autophagy genes,has an important gastric cancer rank relation.
Conclusion: Cucurbitacins D, E, and I purified fromfruits upregulateand induce sub-G1 cell-cycle arrest and cell death in human gastric cancer cell line AGS. Cucurbitacin I effect onmRNA expression is significantly more than that of cucurbitacins E and D.