[Curcumin inhibits growth, induces G1 arrest and apoptosis on human prostatic stromal cells by regulating Bcl-2/Bax].
Zhongguo Zhong Yao Za Zhi. 2008 Aug;33(16):2022-5. PMID: 19086645
Institute of Urology&Nephrology, First Affiliated Hospital, Guangxi Medical Universuity, Nanning 530021, China.
OBJECTIVE: To investigate the effect of curcumin on apoptosis of human prostatic stromal cells.
METHOD: Different concentrations of curcumin were added into culture media system to induce apoptosis of human prostatic stromal cells. The apoptosis was detected by MTT assay, flow cytometry, RT-PCR and TUNEL method.
RESULT: Curcumin at concentrations of 10-40 micromol x L(-1) could inhibit the proliferation of human prostatic stromal cells in a doseand time-dependent manner (P<0.05). Characteristic apoptosis were confirmed by TUNEL RT-PCR manifest downregulation of Bcl-2/Bax (P<0.05). Cell cycle was arrested into G1 phase by curcumin.
CONCLUSION: Curcumin can induce apoptosis in human prostatic stromal cells by down-regulation of Bcl-2/Bax.