[Curcumine inhibits migration and invasion of hepatic stellate cells by reducing MMP-2 expression and activity].
Zhonghua Gan Zang Bing Za Zhi. 2009 Nov;17(11):835-8. PMID: 19958643
Department of General Surgery, Bao'an People's Hospital, Affiliated to Nanfang Medical University, Shenzhen Guangdong 518101, China.
OBJECTIVE: To investigate the molecular mechanism of the inhibitory effect of curcumine on the migration and invasion of hepatic stellate cells (HSC).
METHODS: Rat hepatic stellate cells were cultured and activated with ConA. Matrix metalloproteinase-2 (MMP-2) expression and activity was determined by Western blot and gelatin zymography. Migration and invasion of HSC was assessed by wound healing assay and modified Boyden chamber assay.
RESULTS: Curcumine reduced the level and activity of MMP-2 expression in activated HSC in a dose-dependent manner. When treated with 25, 50 or 100 micromol/L curcumine, the expression of MMP-2 was reduced by 21.8%+/-5.1%, 65.5%+/-9.2% or 87.9%+/-11.5% (P<0.05), and the activity of MMP-2 was also significantly reduced by curcumine. Migration and invasion of activated HSC was also inhibited by curcumine in a dose-dependent way. When treated with 25, 50 or 100 micromol/L curcumine, the migration of activated HSC was reduced by 27.5%+/-5.8%, 54.4%+/-7.6% or 67.1%+/-9.3% (P<0.05), and the invasion of activated HSC was also significantly reduced by curcumine.
CONCLUSION: Curcumine inhibits migration and invasion of activated HSC by reducing MMP-2 expression and activity.