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Abstract Title:

[The inhibitory effect of curcumin on the proliferation of HT-29 colonic cancer cell induced by deoxycholic acid].

Abstract Source:

Zhonghua Nei Ke Za Zhi. 2009 Sep;48(9):760-3. PMID: 20079215

Abstract Author(s):

Bang-mao Wang, Chun-ying Zhai, Wei-li Fang, Xin Chen, Kui Jiang, Yu-ming Wang

Article Affiliation:

Department of Gastroenterology, General Hospital, Tianjin Medical University, Tianjin 300052, China. gi.tmuh@sohu.com

Abstract:

OBJECTIVE: To assess the effect of curcumin on deoxycholic acid (DCA) induced cell proliferation in human HT-29 colon cancer cell line and to discuss its underlying anti-cancer mechanisms.

METHODS: The effect of curcumin on cell proliferation was tested in vitro with MTT assay. RT-PCR was applied to measure cyclooxygenase-2 (COX-2) mRNA transcription. Cellular immunochemical stain was applied to label COX-2 protein expression. Radio immunoassay was applied to assess prostaglandin E(2) (PGE(2)) level.

RESULTS: DCA increased cell proliferation, COX-2 mRNA expression and PGE(2) concentration of HT-29 cell line. Curcumin inhibited DCA-induced cell proliferation of HT-29 cell in a dose and time depended manner. Curcumin may inhibit DCA-induced COX-2 mRNA transcription at 6 hour with more than 5 micromol/L and at 12 hour with more than 0.5 micromol/L. However, Curcumin may decrease DCA-induced COX-2 expression and PGE(2) synthesis at 24 - 48 hour with more than 1 micromol/L and at 6-12 hour with more than 5 micromol/L.

CONCLUSIONS: Curcumin may inhibit HT-29 cell proliferation, COX-2 mRNA transcription, COX-2 protein expression and PGE(2) synthesis induced by DCA in HT-29 cell line. These data provide new insights into the mechanism of its anti-cancer properties.

Study Type : In Vitro Study

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