Abstract Title:

[Relationship between copper injury and apoptosis and the effect of curcumin on copper-injured BRL cells].

Abstract Source:

Psychiatry Res. 1999 Nov 29;88(3):227-32. PMID: 18082042

Abstract Author(s):

Xiao-Hua Wan, Xiao-Ping Luo

Article Affiliation:

Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.


OBJECTIVE: To study the relationship between copper-induced apoptosis and reactive oxygen species (ROS) in BRL cells and the effect of curcumin, a plant-derived polyphenol, on copper-injured BRL cells.

METHODS: BRL cells were treated with CuSO4 (100 micromol/L) or curcumin + CuSO4. The BRL cells without any treatment were used as controls. Flow cytometry was applied to detect the production of ROS with fluorescent probe DCFH-DA. MTT colorimetry was used to evaluate cell activity. Apoptosis was measured using Hoechst 33258 staining and Annexin V-FITC and propidiumiodide (PI) staining. JNK/SAPK protein level was detected using Western blot.

RESULTS: ROS levels (711.70 +/- 68.33 vs 87.22 +/- 7.58) and apoptosis rate (45.08 +/- 1.87% vs 8.23 +/- 2.56%) of BRL cells reached to a peak after 6 hrs of CuSO4 treatment, which were significantly higher than controls (P<0.01). JNK/SAPK levels increased significantly after 6 hrs of CuSO4 treatment and peaked at 24 hrs of CuSO4 treatment compared with controls (P<0.01). Curcumin pretreatment decreased significantly ROS and JNK/SAPK levels as well as the apoptosis rate when compared with the CuSO4-treated alone group (P<0.01).

CONCLUSIONS: Copper may induce apoptosis of BRL cells. ROS participated in apoptosis induced by copper. Curcumin produced protections on copper-injured BRL cells possibly by anti-oxidation and inhibition of p-JNK expression.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Anti-Apoptotic : CK(2905) : AC(1672)

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