Abstract Title:

Differential protection of pre-, co- and post-treatment of curcumin against hydrogen peroxide in PC12 cells.

Abstract Source:

Hum Exp Toxicol. 2010 May 20. Epub 2010 May 20. PMID: 20488840

Abstract Author(s):

M A Siddiqui, M P Kashyap, V Kumar, V K Tripathi, V K Khanna, S Yadav, A B Pant

Article Affiliation:

Indian Institute of Toxicology Research, Lucknow, India (Council of Scientific&Industrial Research, New Delhi, India).


Pharmacological potential of curcumin was assessed in PC12 cells against hydrogen peroxide (H2O2) exposure. In MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and lactate dehydrogenase (LDH) assays, 24-hour exposure of H2O2 (0.5 mM and above) was found to be cytotoxic. A significant (p<0.001) increase in percentage cell viability was recorded in PC12 cells pretreated with curcumin (25, 50 and 100 mug/mL) for 24 hours prior to H2O2 (0.5 and 1 mM) exposure for 24 hours. Co-exposure to H2O2 and curcumin was also found effective. However, a therapeutic treatment of curcumin for 24 hours after H2O2 exposure to the cells was found ineffective. Differential response of PC12-H2O2 model to curcumin in MTT and LDH assays suggests the utility of these endpoints to sort the drug candidates to study their antioxidant potential.

Study Type : In Vitro Study

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