Curcumin Mitigates Immune-Induced Epithelial Barrier Dysfunction by.
Int J Mol Sci. 2019 Sep 28 ;20(19). Epub 2019 Sep 28. PMID: 31569415
Fábia Daniela Lobo de Sá
() is the most common cause of foodborne gastroenteritis worldwide. The bacteria induce diarrhea and inflammation by invading the intestinal epithelium. Curcumin is a natural polyphenol from turmeric rhizome of, a medical plant, and is commonly used in curry powder. The aim of this study was the investigation of the protective effects of curcumin against immune-induced epithelial barrier dysfunction ininfection. The indirect-induced barrier defects and its protection by curcumin were analyzed in co-cultures with HT-29/B6-GR/MR epithelial cells together with differentiated THP-1 immune cells. Electrophysiological measurements revealed a reduction in transepithelial electrical resistance (TER) in infected co-cultures. An increase in fluorescein (332 Da) permeability in co-cultures as well as in the germ-free IL-10mouse model afterinfection was shown. Curcumin treatment attenuated the-induced increase in fluorescein permeability in both models. Moreover, apoptosis induction, tight junction redistribution, and an increased inflammatory response-represented by TNF-α, IL-1β, and IL-6 secretion-was observed in co-cultures after infection and reversed by curcumin. In conclusion, curcumin protects against indirect-triggered immune-induced barrier defects and might be a therapeutic and protective agent in patients.