Curcumin reverse multidrug resistance of osteosarcoma cells. - GreenMedInfo Summary
[Empirical study of reverse effect on mediated multidrug resistance of U-2OS/ADM cells with curcumin in vitro].
Wei Sheng Yan Jiu. 2011 Jan;40(1):103-6. PMID: 21434326
Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China. Xiao_Yang168@sina.com
OBJECTIVE: To investigate the reversion of P-gp mediated multidrug resistance of U-2OS/ADM cells with curcumin in vitro.
METHODS: With doxorubicin as induction drugs, human osteosarcoma cell line U-2OS was induced object, multi-drug resistant of human osteosarcoma cell line model (U-2OS/ADM) were established by high-dose method, The before and after reversal efficacy of curcumin for U-2OS/ADM cells to chemotherapeutic drugs were measured by MTT assay. The effects of curcumin on Rh-123 uptake and efflux were analyzed by flow cytometer.
RESULTS: (1) MTT demonstrates curcumin (20 micromol/L) can increase the cytotoxicity of Adriamycin to U-2OS/ADM cells. (2) The result of FCM shows that Curcumin can increase the accumulation of Rh-123 and increase the cytotoxicity of Adriamycin to U-2OS/ADM cells in a dose-dependent manner.
CONCLUSION: The reversal mechanism of curcumin was blocked the function of P-gp in U-2OS/ADM cellular membrane.