Pinus Densiflora Bark Extract (Pinexol) Decreased Adiposity in Mice by Down-Regulation of Hepatic de novo Lipogenesis and Adipogenesis in White Adipose Tissue.
J Microbiol Biotechnol. 2017 Jan 12. Epub 2017 Jan 12. PMID: 28081360
PineXol, extracted from Korean red pine bark, has beneficial effects, such as antioxidant, anti3 inflammatory, and anti-lipogenic activities in vitro. We tested the hypothesis that PineXol supplementation could have anti-obesity effects on mice fed a high-fat diet (HFD). Four-week old male C57BL/6 mice were fed normal chow (18% kcal from fat) or HFD (60% kcal from fat). HFD-fed animals were also subjected to PineXol treatment at a dose of 10 or 50 mg/kg body weight (BW) (PX10 or PX50, respectively) body weight. Body weight and body fat mass in the PX50 group were statistically lower than those in the HFD group (P<0.05 and P<0.001, respectively). The concentration of hepatic triglycerides, total cholesterol, and low-density lipoprotein cholesterol were reduced in the PX50 group compared to HFD group (P<0.01). Acetyl CoA carboxylase (P<0.01), elongase of very long chain fatty acids 6 (P<0.01), stearoyl CoA desaturase 1 (P<0.05), microsomal triglyceride transfer protein (P<0.01), and sterol regulatory element-binding protein 1 (P<0.05) were significantly decreased in the PX50 group compared with that in the HFD group. In the white adipose tissue, CCAAT-enhancer-binding protein alpha (P<0.05), peroxisome proliferator-activated receptor gamma (P<0.001), and perilipin (P<0.01) were decreased in the PX50 group compared with those in the HFD group. Therefore, the current study implies the potential of PineXol for the prevention and/or amelioration of obesity, in part, by inhibition of both hepatic lipid synthesis and adipogenesis in the white adipose tissue.