Cyanidin-3-glucoside inhibits amyloidβ-induced neuronal cell death in cultured rat hippocampal neurons.
Korean J Physiol Pharmacol. 2018 Nov ;22(6):689-696. Epub 2018 Oct 25. PMID: 30402029
Ji Seon Yang
Increasing evidence implicates changes in [Ca]and oxidative stress as causative factors in amyloid beta (Aβ)-induced neuronal cell death. Cyanidin-3-glucoside (C3G), a component of anthocyanin, has been reported to protect against glutamate-induced neuronal cell death by inhibiting Caand Znsignaling. The present study aimed to determine whether C3G exerts a protective effect against Aβ-induced neuronal cell death in cultured rat hippocampal neurons from embryonic day 17 fetal Sprague-Dawley rats using MTT assay for cell survival, and caspase-3 assay and digital imaging methods for Ca, Zn, MMP and ROS. Treatment with Aβ(20µM) for 48 h induced neuronal cell death in cultured rat pure hippocampal neurons. Treatment with C3G for 48 h significantly increased cell survival. Pretreatment with C3G for 30 min significantly inhibited Aβ-induced [Zn]increases as well as [Ca]increases in the cultured rat hippocampal neurons. C3G also significantly inhibited Aβ-induced mitochondrial depolarization. C3G also blocked the Aβ-induced formation of ROS. In addition, C3G significantly inhibited the Aβ-induced activation of caspase-3. These results suggest that cyanidin-3-glucoside protects against amyloidβ-induced neuronal cell death by reducing multiple apoptotic signals.