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Article Publish Status: FREE
Abstract Title:

Cytotoxic activity of caffeic acid and gallic acid against MCF-7 human breast cancer cells: Anandstudy.

Abstract Source:

Avicenna J Phytomed. 2019 Nov-Dec;9(6):574-586. PMID: 31763216

Abstract Author(s):

Hasan Rezaei-Seresht, Hamid Cheshomi, Farahnaz Falanji, Fatemeh Movahedi-Motlagh, Maryam Hashemian, Erfan Mireskandari

Article Affiliation:

Hasan Rezaei-Seresht

Abstract:

Objective: Phenolic compounds have been considered inhibitors of various cancers.

Material and Methods: In this study, caffeic acid and gallic acid were appraised for their possible effects on apoptotic genes expression in a breast cancer cell line. We also evaluated ligand interaction and ligand binding with estrogen receptor alpha by molecular docking. To determine half maximal inhibitory concentration, MCF-7 cells were treated with different concentrations of caffeic acid and gallic acid by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Furthermore, morphological changes in cells and alterations in,andgene expression were studied by real-time RT-PCR. Also, protein network and different interactions between the desired genes were analyzed using GeneMANIA database.

Results: Evaluation of cell survival by MTT assay revealed that the half-maximal inhibitory concentration values for caffeic acid and gallic acid against MCF-7 cells, were 159 and 18µg/ml, respectively. These compounds were found to affect,andgene expression; this alteration in gene expression probably occurred along with the activation of intrinsic apoptotic signaling pathway.

Conclusion: Via apoptosis induction, caffeic acid and gallic acid have induce toxic effects and morphological changes in breast cancer cells, suggesting their possible future application as antitumor agents.

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