Cytotoxic effect ofextract on human colon cancer cells through repression of telomerase expression.
J Pharm Anal. 2018 Dec ;8(6):394-399. Epub 2017 Dec 15. PMID: 30595946
is a member of the genusthat has long been used for medical objectives. Several studies have reported cytotoxic effects of differentspecies, but there has been only limited investigation of the cytotoxic effect of. The purpose of the current study was to examine the potential effect ofextract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer (WiDr) cells. The cytotoxic effect of the extract was determined using MTT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential (MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR.extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells.extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest thatmay have considerable potential for development as a novel natural product-based anticancer agent.