Article Publish Status: FREE
Abstract Title:

Cytotoxic effect ofextract on human colon cancer cells through repression of telomerase expression.

Abstract Source:

J Pharm Anal. 2018 Dec ;8(6):394-399. Epub 2017 Dec 15. PMID: 30595946

Abstract Author(s):

Ibrahim Turan, Selim Demir, Kagan Kilinc, Serap Ozer Yaman, Sema Misir, Hanife Kara, Berna Genc, Ahmet Mentese, Yuksel Aliyazicioglu, Orhan Deger

Article Affiliation:

Ibrahim Turan


is a member of the genusthat has long been used for medical objectives. Several studies have reported cytotoxic effects of differentspecies, but there has been only limited investigation of the cytotoxic effect of. The purpose of the current study was to examine the potential effect ofextract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer (WiDr) cells. The cytotoxic effect of the extract was determined using MTT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential (MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR.extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells.extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest thatmay have considerable potential for development as a novel natural product-based anticancer agent.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.