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Article Publish Status: FREE
Abstract Title:

Demethoxycurcumin Suppresses Human Brain Glioblastoma Multiforme GBM 8401 Cell Xenograft Tumor in Nude Mice.

Abstract Source:

Int J Mol Sci. 2021 May 23 ;22(11). Epub 2021 May 23. PMID: 34071132

Abstract Author(s):

Yi-Ping Huang, Yi-Shih Ma, Chao-Lin Kuo, Ching-Lung Liao, Po-Yuan Chen, Shu-Fen Peng, Fei-Ting Hsu, Kuang-Chi Lai

Article Affiliation:

Yi-Ping Huang

Abstract:

Demethoxycurcumin (DMC), a derivate of curcumin, has been shown to induce apoptotic cell death in human glioblastoma multiforme GBM 8401 cells via cell cycle arrest and induction of cell apoptosis. However, there is no report showing DMC suppresses glioblastoma multiforme cells. In the present study, we investigated the effects of DMC on GBM8401 cells. At first, we established a luciferase-expressing stable clone named GBM 8401/. Second, mice were inoculated subcutaneously with GBM 8401/cells to generate a xenograft tumor mice model. After inoculation, tumor volume reached 100-120 mm, and all mice were randomly divided into three groups: Group I was treated with 110µL phosphate-buffered solution (PBS) containing 0.1% dimethyl sulfoxide, Group II with 30 mg/kg of DMC, and Group III with 60 mg/kg of DMC. Mice from each group were given the oral treatment of DMC by gavage for 21 days. The body weight and tumor volume were recorded every 3 days. DMC significantlydecreased the tumor volumes, and 60 mg/kg treatment showed a higher decrease in tumor volumes than that of 30 mg/kg, However, DMC did not affect the body weights. The photons emitted from mice tumors were detected with Xenogen IVIS imaging system, DMC at both doses decreased the total photon flux and 60 mg/kg treatment of DMC has low total photon flux than that of 30 mg/kg. The tumor volumes and weights in 60 mg/kg treatment of DMC were lower than that of 30 mg/kg. Immunohistochemical analysis was used to measure protein expression of tumors and results showed that DMC treatment led to lightly staining with anti-Bcl-2 and -XIAP and 60 mg/kg treatment of DMC has lighter staining with anti-Bcl-2 and -XIAP than that of 30 mg/kg. The higher dose (60 mg/kg) of DMC has higher signals of cleaved-caspase-3 than that of the lower dose (30 mg/kg). Furthermore, the hematoxylin and eosin (H&E) staining of liver tissues showed no significant difference between DMC-treated and control-groups. Overall, these observations showed that DMC suppressed tumor propertiesand DMC may be used against human glioblastoma multiforme in the future.

Study Type : Animal Study

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