Dietary inclusion of royal jelly modulates gene expression and activity of oxidative stress enzymes in zebrafish.
J Enzyme Inhib Med Chem. 2021 Dec ;36(1):885-894. PMID: 33752574
Here we investigated the effects of different levels of royal jelly in zebrafish () diets [0.0% (D1); 0.1% (D2); 0.4% (D3); 1.6% (D4) vs 6.4% (D5)] on the activity and expression profiles of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and glutathione S-transferase. Muscle, liver and kidney tissue samples were obtained from fish fed during 8 weeks. In these tissues, enzyme activity was determined by means of spectrophotometer and gene expression by quantitative real-time PCR. mRNA levels of the enzymes were elevated in almost all diet groups compared to the control (D1). It was determined that enzyme activities were also increased ingeneral by supplementation of royal jelly although some decreases were also observed. However, the significant correlation between gene expression and enzyme activity was not observed in all tissues. It was concluded that main regulation occurs with post-translational modifications although effectsat transcriptomic level demonstrated a snap variation.