Dietary unsaponificable fraction from extra virgin olive oil supplementation attenuates acute ulcerative colitis in mice.
Eur J Pharm Sci. 2012 Dec 10. Epub 2012 Dec 10. PMID: 23238173
Department of Pharmacology, School of Pharmacy, University of Seville.
Extra virgin olive oil (EVOO) has demonstrated immunomodulatory and anti-inflammatory properties in murine experimental ulcerative colitis (UC). In addition to its high monounsaturated fatty acid content, evidences have accumulated on the favorable properties of minor, although highly bioactive, components present in the unsaponifiable fraction (UF). The present study was designed to evaluate the effects of dietary EVOO´s UF supplementation on acute UC. C57BL/6 mice were fed from weaning with sunflower oil (SD), EVOO diet and UF-enriched SD at 5% oil (SD+UF). After 30 days, mice were exposed to 3% dextran sulphate sodium (DSS) for 5 days developing acute colitis. After 4 days of DSS removal, animals were sacrificed and colons were histological and biochemically processed. Disease activity index and microscopic damage score were significantly improved in EVOO and SD+UF dietary groups versus SD group. In addition, both dietary treatments significantly induced decreases in MCP-1 and TNF-α levels, iNOS and COX-2 overexpression and p38 MAPKs activation in colon mucosa. Moreover, an up-regulation of IκB expression was also observed after feeding the animals with both diets. However, no statistically differences between data from mice fed with EVOO or UF+SD diets were observed. Dietary enrichment with EVOO's UF reduces the damage in acute colitis model, alleviating the oxidative events and returning pro-inflammatory proteins expression to basal levels probably through p38MAPK and NF-κB signalling pathways. EVOO's UF diet might provide a basis for developing a new strategy in dietary supplementationfor the prevention of UC.