Diphlorethohydroxycarmalol, isolated from the brown algae Ishige okamurae, protects against radiation-induced cell damage in mice.
Food Chem Toxicol. 2011 Apr;49(4):864-70. Epub 2010 Dec 14. PMID: 21163321
Department of Anatomy, College of Medicine, Jeju National University, Jeju 690-756, South Korea; Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, South Korea.
The aim of this study was to evaluate the radioprotective effects of diphlorethohydroxycarmalol (DPHC), isolated from the brown algae Ishige okamurae, in mice subjected to gamma irradiation. DPHC significantly decreased the level of radiation-induced intracellular reactive oxygen species in cultured Chinese hamster lung fibroblast (V79-4) cells (p<0.05), enhanced cell viability that decreased after exposure toγ-rays, and reduced radiation-induced apoptosis in the V79-4 cells. Pretreatment with DPHC (100mg/kg) in mice prior to irradiation significantly protected the intestinal crypt cells in the jejunum (p<0.01) and maintained villi height (p<0.01), compared with those of the vehicle-treated irradiated group. Mice pretreated with DPHC also exhibited dose-dependent increases in the bone marrow cell viability. The dose-reduction factor for gamma irradiation in the DPHC-pretreated mice was 2.05 at 3.5days after irradiation. These results suggest that DHPC plays a role in protecting cells from irradiation-induced apoptosis, through the scavenging of reactive oxygen species in vitro, and that DPHC significantly protected intestinal progenitor cells and bone marrows cells that were decreased by gamma irradiation in vivo.