Differential effects of natural and synthetic vitamin E on gene transcription in murine T lymphocytes.
Arch Biochem Biophys. 2010 Mar 1;495(1):49-55. Epub 2009 Dec 21. PMID: 20026030
Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul, South Korea.
Mice were supplemented with low and high doses of natural and synthetic vitamin E, T cells from the spleen isolated and stimulated with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes assessed by gene array experiments. The data obtained indicate significant qualitative and quantitative differences between the two vitamin forms in regulating gene expression in response to T-cell stimulation. Marker genes have been found whose expression can be considered significant in establishing the level of, and response to vitamin E for both natural and synthetic vitamin E supplementation; unique markers for synthetic vitamin E supplementation and unique markers for natural vitamin E supplementation have been identified.