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Abstract Title:

Electronic cigarettes induce DNA strand breaks and cell death independently of nicotine in cell lines.

Abstract Source:

Oral Oncol. 2015 Nov 4. Epub 2015 Nov 4. PMID: 26547127

Abstract Author(s):

Vicky Yu, Mehran Rahimy, Avinaash Korrapati, Yinan Xuan, Angela E Zou, Aswini R Krishnan, Tzuhan Tsui, Joseph A Aguilera, Sunil Advani, Laura E Crotty Alexander, Kevin T Brumund, Jessica Wang-Rodriguez, Weg M Ongkeko

Article Affiliation:

Vicky Yu

Abstract:

OBJECTIVES: Evaluate the cytotoxicity and genotoxicity of short- and long-term e-cigarette vapor exposure on a panel of normal epithelial and head and neck squamous cell carcinoma (HNSCC) cell lines.

MATERIALS AND METHODS: HaCaT, UMSCC10B, and HN30 were treated with nicotine-containing and nicotine-free vapor extract from two popular e-cigarette brands for periods ranging from 48h to 8weeks. Cytotoxicity was assessed using Annexin V flow cytometric analysis, trypan blue exclusion, and clonogenic assays. Genotoxicity in the form of DNA strand breaks was quantified using the neutral comet assay andγ-H2AX immunostaining.

RESULTS: E-cigarette-exposed cells showed significantly reduced cell viability and clonogenic survival, along with increased rates of apoptosis and necrosis, regardless of e-cigarette vapor nicotine content. They also exhibited significantly increased comet tail length and accumulation ofγ-H2AX foci, demonstrating increased DNA strand breaks.

CONCLUSION: E-cigarette vapor, both with and without nicotine, is cytotoxic to epithelial cell lines and is a DNA strand break-inducing agent. Further assessment of the potential carcinogenic effects of e-cigarette vapor is urgently needed.

Study Type : In Vitro Study
Additional Links
Adverse Pharmacological Actions : Genotoxic : CK(201) : AC(78)

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