Effect ofvar.Fruit on Biofilm Formed by.
Biomed Res Int. 2019 ;2019:3547858. Epub 2019 Dec 10. PMID: 31886204
is an important pathogenic anaerobic bacterium that causes aspiration pneumonia. This bacterium frequently forms biofilms in the oral cavity and in respiratory tract-associated medical devices. Bacterial colonization that occurs in association with this biofilm formation is the main reason for incurable aspiration pneumonia. Thevar.(LCE) fruit has been used in folk medicine in Hokkaido, the northern part of Japan. The aim of this study was to elucidate one of the antimicrobial mechanisms of LCE methanol extract (LCEE)-the inhibitory effect of LCEE on biofilm formation byOur results show that LCEE significantly reduced biofilm formation by three differentisolates in a concentration- and time-dependent manner that were quantified by the adsorption of safranin red. When LCEE was added to biofilms already formed by, LCEE did not degrade the biofilm. However, treatment with LCEE significantly promoted the removal of existing biofilm by vibration compared to that of control. We also confirmed biofilm formation in LCEE-treatedin tracheal tubes using scanning electron microscopic (SEM) analysis. Cyanidin 3-O-glucoside (C3G), one of the components of LCE, also inhibited the formation of biofilm byin a concentration-dependent manner. Our results reveal that LCEE may be an effective antibacterial substance for-induced aspiration pneumonia because of its role in the suppression of bacterial biofilm formation in the oral cavity.