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Article Publish Status: FREE
Abstract Title:

Effect of microvesicles fromcontaining miRNA on proliferation and apoptosis in tumor cell lines.

Abstract Source:

Cell Death Discov. 2020 ;6:43. Epub 2020 Jun 4. PMID: 32550010

Abstract Author(s):

Marina Potestà, Valentina Roglia, Marialaura Fanelli, Elisa Pietrobono, Angelo Gismondi, Simone Vumbaca, Rick Gildas Nguedia Tsangueu, Antonella Canini, Vittorio Colizzi, Sandro Grelli, Antonella Minutolo, Carla Montesano

Article Affiliation:

Marina Potestà

Abstract:

Human microvesicles are key mediators of cell-cell communication. Exosomes function as microRNA transporters, playing a crucial role in physiological and pathological processes. Plant microvesicles (MVs) display similar features to mammalian exosomes, and these MVs might enhance plant microRNA delivery in mammals. Considering that plant microRNAs have been newly identified as bioactive constituents in medicinal plants, and that their potential role as regulators in mammals has been underlined, in this study, we characterized MVs purified fromseeds aqueous extract (MOES MVs) and used flow cytometry methods to quantify the ability to deliver their content to host cells. The microRNAs present in MOES MVs were characterized, and through a bioinformatic analysis, specific human apoptosis-related target genes of plant miRNAs were identified. In tumor cell lines, MOES MVs treatment reduced viability, increased apoptosis levels associated with a decrease in B-cell lymphoma 2 protein expression and reduced mitochondrial membrane potential. Interestingly, the effects observed with MOES MVs treatment were comparable to those observed with MOES treatment and transfection with the pool of small RNAs isolated from MOES, used as a control. These results highlight the role of microRNAs transported by MOES MVs as natural bioactive plant compounds that counteract tumorigenesis.

Study Type : In Vitro Study

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