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Article Publish Status: FREE
Abstract Title:

Emodin ininhibits oxidative stress in the liver via AMPK with Hippo/Yap signalling pathway.

Abstract Source:

Pharm Biol. 2020 Dec ;58(1):333-341. PMID: 32306810

Abstract Author(s):

Eun Hye Lee, Su Youn Baek, Ji Young Park, Young Woo Kim

Article Affiliation:

Eun Hye Lee

Abstract:

Emodin is a compound inLinne (Polygonaceae) that has been reported to exert anti-inflammatory, antibacterial, and antiallergic effects.Oxidative stress is a causative agent of liver inflammation that may lead to fibrosis and hepato-carcinoma. In this study, we investigated the antioxidant effects of emodin and its mechanism.We used the hepatocyte stimulated by arachidonic acid (AA) + iron cotreatment and the C57B/6 mice orally injected with acetaminophen (APAP, 500 mg/kg, 6 h), as assessed by immunoblot and next generation sequencing (NGS). Emodin was pre-treated in hepatocyte (3 ∼ 30 μM) for 1 h before AA + iron, and in mice (10 and 30 m/kg, P.O.) for 3 days before APAP., emodin treatment inhibited the cell death induced by AA + iron maximally at a dose of 10 μM (EC>3 μM). In addition, emodin attenuated the decrease of anti-apoptotic proteins, and restored mitochondria membrane potential as mediated by the liver kinase B1 (LKB1)-AMP-activated protein kinase (AMPK) pathway. LKB1 mediated AMPK activation was verified using the LKB1 deficient cell line, HeLa. Emodin (10 μM; after 10 min) also induced the phosphorylation of Yes-associated protein 1 (YAP1), the main downstream target of the Hippo signalling pathway that mediated oxidative stress or the ROS-initiated signalling pathway.the oral treatment of emodin (10 and 30 m/kg, 3 days) decreased APAP-induced hepatic damage, as indicated by decreases in antioxidant genes as well as tissue damage.Our results show that emodin inhibits oxidative liver injury via the AMPK/YAP mediated pathway.

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