[Epigallocatechin gallate inducesgene demethylation to promote acute myeloid leukemia cell apoptosisby regulating p19-p53-p21signaling pathway].
Nan Fang Yi Ke Da Xue Xue Bao. 2020 Sep 30 ;40(9):1230-1238. PMID: 32990229
OBJECTIVE: To investigate the mechanism by which epigallocatechin gallate (EGCG) inducesgene demethylation and promotes the apoptosis of acute myeloid leukemia KG-1 and THP-1 cell lines.
METHODS: KG-1 and THP-1 cells treated with 25, 50, 75, 100 or 150μg/mL EGCG for 48 h were examined forgene methylation using MSP and for cell proliferation using MTT assay. The changes in cell cycle and apoptosis of the two cell lines after treatment with EGCG for 48 h were detected using flow cytometry. The mRNA and protein expressions of DNMT1, CHD5, p19, p53 and p21in the cells were detected using RT-quantitative PCR and Western blot.
RESULTS: EGCG dose-dependently reversed hypermethylation ofgene and reduced the cell viability in both KG-1 and THP-1 cells (<0.05). EGCG treatment caused obvious cell cycle arrest in G1 phase, significantly increased cell apoptosis, downregulated the expression of DNMT1 and upregulated the expressions of CHD5, p19, p53 and p21in KG-1 and THP-1 cells (<0.05).
CONCLUSIONS: EGCG reduces hypermethylation ofgene in KG-1 and THP-1 cells by downregulating DNMT1 to restore its expression, which results in upregulated expressions of p19, p53 and p21and induces cell apoptosis.